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hybridization buffer  (Molecular Instruments)
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Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hcr hybridization buffer  (Molecular Instruments)
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Molecular Instruments hcr hybridization buffer
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probe hybridization buffer  (Molecular Instruments)
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Molecular Instruments probe hybridization buffer
(A) Example image of Gad2 expression in the adult mouse habenula. Scale bar, 100 μm. Allen Mouse Brain Atlas, https://mouse.brain-map.org/experiment/show/79591669 . (B) UMAP projection of single-cell RNA sequencing from Allen Institute ABC Atlas, filtered for neurons from the habenula and re-clustered using the Leiden algorithm. (C) UMAP projection in B, pseudocolored by the normalized expression of Gad2 . (D) Overlap of Gad2 and Oprm1 expression in the same scRNAseq data set. Percentages indicate the proportion of LHb neurons in that condition; 30% of neurons express neither gene. (E) Example image of a double in situ <t>hybridization</t> for Fos and Gad2 mRNA in the habenula during spontaneous withdrawal. Scale bar, 50 μm. (F) Mean density of Fos + neurons in the LHb. **p< 0.01 by Mann-Whitney Test. (G) Recruitment Index, calculated the difference in probabilities of Gad2 expression in Fos + and Fos - cells, divided by the sum of those probabilities. Mann-Whitney test, p<0.05. (H) Mean density of Gad2 + neurons in the LHb in saline control and spontaneous withdrawal. Open circles indicate female, filled circles indicate male. Error bars indicate standard deviation. n= 61 sections from 14 mice.
Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hcrtm probe hybridization buffer v3 0 molecular instruments hcrtm probe hybridization buffer  (Molecular Instruments)
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Molecular Instruments hcrtm probe hybridization buffer v3 0 molecular instruments hcrtm probe hybridization buffer
(A) Example image of Gad2 expression in the adult mouse habenula. Scale bar, 100 μm. Allen Mouse Brain Atlas, https://mouse.brain-map.org/experiment/show/79591669 . (B) UMAP projection of single-cell RNA sequencing from Allen Institute ABC Atlas, filtered for neurons from the habenula and re-clustered using the Leiden algorithm. (C) UMAP projection in B, pseudocolored by the normalized expression of Gad2 . (D) Overlap of Gad2 and Oprm1 expression in the same scRNAseq data set. Percentages indicate the proportion of LHb neurons in that condition; 30% of neurons express neither gene. (E) Example image of a double in situ <t>hybridization</t> for Fos and Gad2 mRNA in the habenula during spontaneous withdrawal. Scale bar, 50 μm. (F) Mean density of Fos + neurons in the LHb. **p< 0.01 by Mann-Whitney Test. (G) Recruitment Index, calculated the difference in probabilities of Gad2 expression in Fos + and Fos - cells, divided by the sum of those probabilities. Mann-Whitney test, p<0.05. (H) Mean density of Gad2 + neurons in the LHb in saline control and spontaneous withdrawal. Open circles indicate female, filled circles indicate male. Error bars indicate standard deviation. n= 61 sections from 14 mice.
Hcrtm Probe Hybridization Buffer V3 0 Molecular Instruments Hcrtm Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Example image of Gad2 expression in the adult mouse habenula. Scale bar, 100 μm. Allen Mouse Brain Atlas, https://mouse.brain-map.org/experiment/show/79591669 . (B) UMAP projection of single-cell RNA sequencing from Allen Institute ABC Atlas, filtered for neurons from the habenula and re-clustered using the Leiden algorithm. (C) UMAP projection in B, pseudocolored by the normalized expression of Gad2 . (D) Overlap of Gad2 and Oprm1 expression in the same scRNAseq data set. Percentages indicate the proportion of LHb neurons in that condition; 30% of neurons express neither gene. (E) Example image of a double in situ hybridization for Fos and Gad2 mRNA in the habenula during spontaneous withdrawal. Scale bar, 50 μm. (F) Mean density of Fos + neurons in the LHb. **p< 0.01 by Mann-Whitney Test. (G) Recruitment Index, calculated the difference in probabilities of Gad2 expression in Fos + and Fos - cells, divided by the sum of those probabilities. Mann-Whitney test, p<0.05. (H) Mean density of Gad2 + neurons in the LHb in saline control and spontaneous withdrawal. Open circles indicate female, filled circles indicate male. Error bars indicate standard deviation. n= 61 sections from 14 mice.

Journal: bioRxiv

Article Title: Opioid withdrawal engages a habenular subpopulation responsive to aversive states

doi: 10.64898/2026.04.29.721196

Figure Lengend Snippet: (A) Example image of Gad2 expression in the adult mouse habenula. Scale bar, 100 μm. Allen Mouse Brain Atlas, https://mouse.brain-map.org/experiment/show/79591669 . (B) UMAP projection of single-cell RNA sequencing from Allen Institute ABC Atlas, filtered for neurons from the habenula and re-clustered using the Leiden algorithm. (C) UMAP projection in B, pseudocolored by the normalized expression of Gad2 . (D) Overlap of Gad2 and Oprm1 expression in the same scRNAseq data set. Percentages indicate the proportion of LHb neurons in that condition; 30% of neurons express neither gene. (E) Example image of a double in situ hybridization for Fos and Gad2 mRNA in the habenula during spontaneous withdrawal. Scale bar, 50 μm. (F) Mean density of Fos + neurons in the LHb. **p< 0.01 by Mann-Whitney Test. (G) Recruitment Index, calculated the difference in probabilities of Gad2 expression in Fos + and Fos - cells, divided by the sum of those probabilities. Mann-Whitney test, p<0.05. (H) Mean density of Gad2 + neurons in the LHb in saline control and spontaneous withdrawal. Open circles indicate female, filled circles indicate male. Error bars indicate standard deviation. n= 61 sections from 14 mice.

Article Snippet: Tissue was washed two times in 5xSSC, then incubated in probe hybridization buffer (Molecular Instruments) for 30 minutes.

Techniques: Expressing, Single Cell, RNA Sequencing, In Situ Hybridization, MANN-WHITNEY, Saline, Control, Standard Deviation

(A) Left, experimental paradigm for acute naloxone-precipitated withdrawal. Right, example images from double in situ hybridization for Fos and Gad2 mRNA in the habenula following naloxone-precipitated withdrawal. Scale bar, 50 μm. (B) Mean density of Fos + neurons in the LHb. Two-Way ANOVA, Naloxone effect, p<0.001. Morphine effect, p=.07; Interaction, p=0.18. Post hoc Fisher’s test. (C) Mean density of Fos + Gad2 + coexpressing neurons in the LHb. Two-Way ANOVA, Naloxone effect, p< 0.01. Morphine effect, p<0.05; Interaction, p=0.12. Post hoc Fisher’s test. (D) Recruitment Index, calculated as the difference in probabilities of Gad2 expression in Fos + and Fos - populations, divided by the sum of those probabilities. ANOVA, Naloxone effect, p<0.05; post hoc Fisher’s test. (E) Experimental paradigm for recording activity from LHb GAD2 neurons during withdrawal. Gad2-Cre::AAV-DIO-GCaMP animals receive an injection of morphine at t = 0 min. Fiber photometry recording begins at t = 90 min. Animals are injected with naloxone or saline at 120 min and photometry recording continues to 150 min. (F) Example recordings from animals before and after the second injection of naloxone or saline. (G) Change in the area under the curve before and after naloxone or saline injection, calculated as AUC 5 min after second injection minus AUC 5 min before second injection. Dots indicate individual animals. Error bars indicate SEM. Two-Way ANOVA, Naloxone effect, *p<0.05; Morphine effect, p=0.43; Interaction, p=0.13. Post hoc Fisher’s test. (H) Histograms of the probability distribution of fiber photometry fluorescence values before (gray) and after (colored) the second injection in each treatment condition. SS, Saline + Saline; SN, Saline + Naloxone; MS, Morphine + Saline; MN, Morphine + Naloxone. n = 14 animals. Error bars indicate SEM. (I) AUC across the duration of photometry recording, calculated as a 5 min moving average of the AUC from injection onset to 25 min after the injection, relative to the AUC 5 minutes before the injection. *p<0.05, ***p<0.001

Journal: bioRxiv

Article Title: Opioid withdrawal engages a habenular subpopulation responsive to aversive states

doi: 10.64898/2026.04.29.721196

Figure Lengend Snippet: (A) Left, experimental paradigm for acute naloxone-precipitated withdrawal. Right, example images from double in situ hybridization for Fos and Gad2 mRNA in the habenula following naloxone-precipitated withdrawal. Scale bar, 50 μm. (B) Mean density of Fos + neurons in the LHb. Two-Way ANOVA, Naloxone effect, p<0.001. Morphine effect, p=.07; Interaction, p=0.18. Post hoc Fisher’s test. (C) Mean density of Fos + Gad2 + coexpressing neurons in the LHb. Two-Way ANOVA, Naloxone effect, p< 0.01. Morphine effect, p<0.05; Interaction, p=0.12. Post hoc Fisher’s test. (D) Recruitment Index, calculated as the difference in probabilities of Gad2 expression in Fos + and Fos - populations, divided by the sum of those probabilities. ANOVA, Naloxone effect, p<0.05; post hoc Fisher’s test. (E) Experimental paradigm for recording activity from LHb GAD2 neurons during withdrawal. Gad2-Cre::AAV-DIO-GCaMP animals receive an injection of morphine at t = 0 min. Fiber photometry recording begins at t = 90 min. Animals are injected with naloxone or saline at 120 min and photometry recording continues to 150 min. (F) Example recordings from animals before and after the second injection of naloxone or saline. (G) Change in the area under the curve before and after naloxone or saline injection, calculated as AUC 5 min after second injection minus AUC 5 min before second injection. Dots indicate individual animals. Error bars indicate SEM. Two-Way ANOVA, Naloxone effect, *p<0.05; Morphine effect, p=0.43; Interaction, p=0.13. Post hoc Fisher’s test. (H) Histograms of the probability distribution of fiber photometry fluorescence values before (gray) and after (colored) the second injection in each treatment condition. SS, Saline + Saline; SN, Saline + Naloxone; MS, Morphine + Saline; MN, Morphine + Naloxone. n = 14 animals. Error bars indicate SEM. (I) AUC across the duration of photometry recording, calculated as a 5 min moving average of the AUC from injection onset to 25 min after the injection, relative to the AUC 5 minutes before the injection. *p<0.05, ***p<0.001

Article Snippet: Tissue was washed two times in 5xSSC, then incubated in probe hybridization buffer (Molecular Instruments) for 30 minutes.

Techniques: In Situ Hybridization, Expressing, Activity Assay, Injection, Saline, Fluorescence